Made in FRANCE with US row materials
Made in FRANCE with US row materials
"Your Confidence is Our Satisfaction"
"Your Confidence is Our Satisfaction"

TB-SPRINT

TB-SPRINT
TB_SPRINT_ENG.pdf
Document Adobe Acrobat [711.1 KB]

The simultaneous spoligotypingrpoBkatGinhA loci SNPs typing is a brand-new technique to  control and survey multidrug-resistant TB emergence, show to be 100% correlated to rpoB and katG hot-spot sequencing, that can be run in 3h on a single Eppendorf tube. 

The technique uses the “Dual Priming Oligonucleotide” principle (DPO) and a 8 primers multiplexed  PCR. It can also be run in two experiments, i.e. a 16-Plex RINT (Rifampin, Isoniazid Typing), and a 43-Plex SPOL experiment. The two modules can be purchased separately.

 

It consists in its full version in a 59-Plex assay (to run in a single read on Luminex 200® or BioPlex, whereas the MagPix version requires two reads).

 

The 59-Plex includes the detection of 43 spacers, 3 wild-type sequences on rpoB, 5 prevalent SNPs prevalent on rpoB  (at position 516 (mut1), 526 (mut1, mut2), 531 (mut1, mut2), two “spanning” probes (spa_wt1, spa_wt2) that allow the indirect mutation detection of untargeted SNPs at the other positions of the rpoB Hot-Spot, and katG (wt, mut 1, mut2) and inhA (wt, mut1, mut2).

 

The technique was developed and validated on culture extracted DNA of MDR-TB cases and can also be used on clinical samples.

 

 

for Research Use Only

French version
Fiche_BMX_ TB_SPRINT_Recto_F.pdf
Document Adobe Acrobat [492.2 KB]
Italian version
Fiche_BMX_TB_SPRINT_Recto_IT.pdf
Document Adobe Acrobat [369.6 KB]

How to join us?

info@beamedex.com

order@beamedex.com

 

Beamedex SAS
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Université Paris Sud

91400 Orsay

Téléphone :

+33 1 69 15 46 46 or 46 48

 

RCS Evry:

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Director of Publication : Christophe Sola

Website Administration :

Pasquale Cutolo, Clement Ripoll

News

Coming soon : general review on the ESM congress (held in Sibenik from June 25th till June 28th 2017)

 

Why should you switch from membrane to microbead-based spoligotyping ?

 

A new Allele call Macro for TB-RINT users is available