« STM-CRISPOL » (genotyping of Salmonella enterica CRISPR locus) is a new surveillance technique for food-borne diseases caused by Salmonella enterica serovar typhimurium (one of the main serovars responsible of infections), which was invented at the Institut Pasteur by Dr. FX Weill (French patent (no. FR07/09188) international (no. PCT/IB2008/004004).
This technique is directly inspired by the Mycobacterium tuberculosis “spoligotyping” technique with specific Salmonella CRISPR probes discovered at Institut Pasteur, Paris. The technique consist in a 72-Plex assay on Luminex® microbead-based systems, including detection of 68 spacers and 4 SNPs variants on some spacers.
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Coming soon : general review on the ESM congress (held in Valencia from June 30th till July 3rd 2019)