Five kits have been developed and are ready for use, they are based on the coupling of specific probes to microspheres. Samples are amplified by PCR reactions, and hybridization/detection is done on Luminex systems. Targets are either SNPs, deletion regions, or spacers of CRISPR loci.
- TB-SPOL supports the "spoligotyping" technique on Mycobacterium tuberculosis complex DNA (43 plex for Luminex 200 or Magpix, on magnetic or polystyren microspheres).
- TB-SPRINT supports the simultaneous Dual Priming Oligonucleotide (DPO) based spoligotyping and main Rifampin and Isoniazid SNPs characterization on Mycobacterium tuberculosis complex DNA to monitor and control Multi-Drug-Resistant Tuberculosis spreading (59 plex for Luminex 200 or 43 plex + 16 plex for Magpix, on magnetic or polystyren microspheres)
- STM-CRISPOL supports the Salmonella enterica serovar typhimurium subtyping using CRISPR genetic diversity to early detect potential outbreaks linked to this food-borne pathogen agent (72 plex, for Luminex 200 on magnetic or polystyren microspheres).
TB- ULTRA 15-Plex for second-lane TB drug-resistance, 34-Plex for first and second-lane TB drug-resistance). TB-SPRINT+ QUINOLONES (gyrA), AMINOGLYCOSIDES, (rrs, eis) and ETHAMBUTOL (embB) genotypic resistance typing (34-Plex and 43-Plex, i.e. 77-Plex in the most extensive version).
Université Paris Sud
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Director of Publication : Christophe Sola
Website Administration :
Pasquale Cutolo, Clement Ripoll
Coming soon : general review on the ESM congress (held in Sibenik from June 25th till June 28th 2017)
Why should you switch from membrane to microbead-based spoligotyping ?
A new Allele call Macro for TB-RINT users is available